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M9550251.TXT
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1995-03-04
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Document 0251
DOCN M9550251
TI Isolation and identification of Encephalitozoon hellem from an Italian
AIDS patient with disseminated microsporidiosis.
DT 9505
AU Scaglia M; Sacchi L; Gatti S; Bernuzzi AM; Polver P de P; Piacentini I;
Concia E; Croppo GP; da Silva AJ; Pieniazek NJ; et al; Laboratory of
Clinical Parasitology, Pavia University-IRCCS San; Matteo, Italy.
SO APMIS. 1994 Nov;102(11):817-27. Unique Identifier : AIDSLINE
MED/95134377
AB Microsporidia are primitive mitochondria-lacking spore-forming
eukaryotic protozoa that infect a wide variety of animals and also
humans. Of the five genera (Encephalitozoon, Enterocytozoon, Septata,
Nosema and Pleistophora) that cause infections in humans, Enterocytozoon
bieneusi, Septata intestinalis, and Encephalitozoon hellem are being
increasingly identified in patients with acquired immunodeficiency
syndrome (AIDS). E. bieneusi causes gastrointestinal disease, S.
intestinalis causes gastrointestinal and disseminated disease, and E.
hellem causes ocular as well as disseminated disease. We have
established in continuous culture a strain of microsporidia isolated
from the urine and throat washings of an Italian AIDS patient and
identified it as Encephalitozoon hellem, based on its ultrastructural
morphology, antigenic pattern, and polymerase chain reaction-amplified
small subunit ribosomal RNA. We believe that this is the first time that
a strain of microsporidia has been isolated from the throat washings of
a patient with microsporidiosis.
DE Adult Animal AIDS-Related Opportunistic Infections/*PARASITOLOGY Base
Sequence Cell Line Cercopithecus aethiops DNA Primers
Encephalitozoon/GROWTH & DEVELOPMENT/*ISOLATION & PURIF/ ULTRASTRUCTURE
Encephalitozoonosis/*COMPLICATIONS/PARASITOLOGY Fluorescent Antibody
Technique Human Immunoblotting Italy Microscopy, Electron
Microscopy, Electron, Scanning Molecular Sequence Data
Pharynx/PARASITOLOGY RNA, Ribosomal/GENETICS Support, Non-U.S. Gov't
Vero Cells JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).